Best Preanalytic Practices

laatst gewijzigd: 29. augustus 2022 | Lablife - Terug naar overzicht

The Centers for Disease Control and Prevention state that 70% of medical decisions depend on laboratory test results.[1] This illustrates the importance and influence of best practice in the preanalytical area on the evaluation in clinical laboratories.

The following are several best practices that should be kept in mind to ensure high quality samples and accurate test results.

Identify the patient

Minimum requirements include the patient’s full name, date of birth and a patient-specific identifier (e.g. ID card, patient wristband)
Confirm identity by soliciting verbal response to open-ended questions requiring specific information
Compare information provided to wristband, which must be attached to the patient, and test requisition
Resolve any discrepancies prior to proceeding with specimen collection

Follow the order of draw

Collect tubes according to the Order of Draw as established in CLSI GP41[2] and according to facility policy.

Preventing under-filled tubes

Push tube forward so stopper is fully penetrated
Hold blood collection tube according to IFU and by pressing the tube with the thumb or finger to ensure complete vaccum draw
Keep tube in place until vacuum is exhausted and blood ceases to flow into tube
Use discard tube if winged collection set is used and coagulation tube is drawn first

Preventing hemolysis

Tourniquet application should not be overly constrictive nor should it exceed one minute
Allow alcohol to dry according to IFU prior to proceeding
Choose appropriate equipment based on circumstance of the draw
Fill tubes completely and mix with recommended number of gentle inversions
If collecting from a vascular access device (VAD), an appropriate transfer device should be used, discard volumes considered and proper procedures for flushing the line should be followed. The discard volume of the VAD is required due to particles from the puncturing process.
Avoid excessive pulling force when using a syringe and follow proper procedure for transfer to evacuated tubes following collection

Preventing fibrin formation

Fill tubes to indicated level to ensure proper blood to additive ratio
Gently invert tubes according to IFU to mix the additive with the sample
Allow serum tubes to clot thoroughly (minimum 30 minutes) in an upright position

Label tubes

Label tubes in presence of patient
Reconfirm patient identification
Place the patient label along the top edge of the tube label such that additive and fill mark remain visible

There may be additional considerations depending on the patient condition and the tests ordered. Another helpful article for more information can be found at https://www.gbo.com/en-us/spotlight/article/the-top-5-phlebotomy-basics-to-know.

[1]Division of Laboratory Systems (DLS). Strengthening Clinical Laboratories. 2018. https://www.cdc.gov/csels/dls/strengthening-clinical-labs.html

[2]Clinical and Laboratory Standards Institute (CLSI). Collection of Diagnostic Venous Blood Specimens. 7th ed. CLSI standard GP41 (ISBN 1-56238-812-6 [Print]; ISBN 1-56238-813-4 [Electronic]). Clinical and Laboratory Standards Institute, 950 West Valley Road, Suite 2500, Wayne, Pennsylvania 19087 USA, 2017.

[3] VACUETTE® Best Preanalytic Practices. Greiner Bio-One North America, Inc.

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