Preguntas frecuentes

Aquí puede encontrar respuestas a sus preguntas sobre Bio-One productos. 

Las preguntas frecuentes le ayudarán a realizar sus actividades diarias con los productos de Greiner Bio-One con incluso mayor facilidad. Describen la secuencia correcta de manipulación y proporcionan consejos valiosos para evitar que se produzcan errores.

Puede buscar específicamente con la función de búsqueda de texto libre para obtener la respuesta correcta a su pregunta; o bien puede explorar a través de las categorías de productos para consultar información adicional.

La página de preguntas frecuentes se actualiza de forma constante con sus preguntas y con las respuestas de los expertos en productos de Greiner Bio-One.

Si no obtiene respuesta a su pregunta, solo tiene que enviárnosla utilizando el formulario.

Se le notificará con un mensaje de correo electrónico tan pronto como se haya publicado una respuesta en línea.

Siempre estamos dispuestos a ayudarle.

No se han encontrado preguntas frecuentes.
Respuesta:

Every Greiner Bio-One medical device product has an GMDN- and EDMA-Code which has to be stated at the EU-accreditation.

Respuesta:

Classification Code:

- EDMA (European Diagnostic Manufacturers Association)

- GMDN (Global Medical Device Nomenclature 

Respuesta:

The 96 well µClear® microplates and 384 well µClear® microplates have a film thickness of 190 µm +/- 10 %.

In the 1536 well microplate with a transparent bottom (µClear®) the film thicknee is 75 µm +/- 10 %. 

UV-Star® microplates generally have a film thickness of 135 µm +/- 10 µm.

Respuesta:

It is not recommended to use cell culture treated plates (TC treated microplates / Cellstar® microplates) in ELISAs. The cell culture treatment (TC treatment) of polystyrene creates highly charged surfaces with high affinity to all kind of polar proteins. Therefore the non specific background in the cell culture treated microplates is hard to control. Additionally, the ability of our immunological products  (Microlon®) to bind proteins consistently and reproducibly is constantly checked in our quality assurance laboratory by means of ELISA.

Respuesta:

Microplates made out of polystyrene do not withstand the high temperatures of the autoclaving process (specified thermal stability of polystyrene = -20°C - + 60°C)

Microplates made out of polypropylene do have a higher temperature tolerance (specified temperature range of polypropylene = -196°C to + 121°C).

All microplates are intended for single use only and autoclaving is not recommended as autoclaving can influence the tight physical dimensions and tolerances of the microplates.

However most of our microplates are as well available in a sterile version and autoclaving is not necessary.

Respuesta:

White microplates are usually used for luminescence measurements and black microplates for fluorescence measurements. The critical properties in these methods, such as background, autofluorescence or crosstalk are considerably improved by the use of black or white pigmented microplates.

Respuesta:

96 well microplates are in available with different well designs:

 

V-bottom: The V stands for the conically tapered well bottom. These microplates are ideally suited for applications in which the entire sample volume must be pipetted of.

 

U-bottom: The U describes the round bottom shape. U-bottom microplates are ideally suited for agglutination tests.

 

F-bottom: The F stands for the flat bottom of the wells. This well type is ideal for precise optical measurements. The measuring light source is not deflected by the well profile.

Respuesta:

The µClear® microplates are clear bottom microplates with a thin polystyrene film bottom (190 µm +/- 20 µm for a 96 and 384 well formats and 75 µm +/- 10 µm for the 1536 well format).

Commonly µClear® microplates are used in cell based assays as they allow the microscopic monitoring of the cell growth. Additionally µlear® microplates have increased sensitivity down to 340 nm due to a reduction in background in transmission measurements.

Respuesta:

Yes! For the determination of nucleic acid and protein concentrations at 260 nm or 280 nm without background interference the UVStar® microplates are an ideal alternative to expensive and fragile quartz glass microplates or cuvettes.

 

Different UVStar® microplates are available:

 

- Standard 96 well UVStar® microplates (Part. No. 655 801)

- Half Area 96 well UVStar® microplates (Part. No. 675 801)

- 384 well UVStar® microplates (Part. No. 781 801)

Respuesta:

The microplate with Cat.-No. 784080 is a 384 well white Small Volume™, solid bottom, cell culture treated, sterile microplate with lid. The plate does not have a clear film it is a solid bottom microplate. Small Volume™ microplates with a clear film bottom (µClear®) are available in the low base design (= references starting with 788 ...) E.g. Reference 788 093 = 384 well, white, Small Volume™, µClear®, cell culture treated, sterile microplate without lid.

The difference between Small Volume™ LoBase and Hi Base design is the height of the plate (Small Volume™ HiBase = 14.4 mm / Small Volume™ LoBase = 7.5 mm) and the position of the wells.
Customer Drawings of both plates can be downloaded from our webpage.

Price inquirys will be answered from our distributors and subsidiaries worldwide.

Respuesta:

No we do not have 48 well glass bottom plates in our product portfolio, but the following glass bottom plates (Sensoplates™ ) are available from Greiner Bio-One GmbH:

24 well glass bottom microplates
# 662892 24 well glass bottom microplates, lid, single wrapped
# 662896 24 well glass bottom microplates, no lid, single wrapped

96 well glass bottom microplates
# 655892 96 well glass bottom microplates, lid, sterile, single wrapped
# 655896 96 well glass bottom microplates, no lid, sterile, single wrapped

384 well glass bottom microplates
# 781892 384 well glass bottom microplates , lid, sterile, single wrapped
# 781896 384 well glass bottom microplates, no lid, sterile, single wrapped
# 781856 384 well glass bottom microplates, extra lobase, no lid, sterile, single wrapped

1536 well glassbottom microplates
# 783892 1536 well glass bottom microplates, lid, sterile, single wrapped
# 783896 1536 well glass bottom microplates, no lid, sterile, single wrapped
# 783856 1536 well glass bottom microplates, extra lobase, no lid, sterile, single wrapped

Respuesta:

For 781 182 the recommended working volume is between 10 and 130 µl - depends on the requirements and applications of the customer.

Detailed informations about most products can be found in the customer drawings and technical data sheets on our website in the product catalogue under the relevant part number. 

Respuesta:

Those products are sterilised by ionising radiation with a Sterility Assurance Level (SAL) of 10-3. The validation of the irradiation dose is according to EN 552, ISO 11137, ANSI/AAMI ST31/ST32.

Respuesta:

If sterility is not a requirement for the assay the non treated med binding plates can be used.
If the assay should be run under sterile conditions sterile plates should be used. If no special sterile microplate is listed in our product catalogue, e,g, for the black or white solid bottom microplates, we recommend to use the high binding plates as these plates are due to the high binding treatment sterile as well .

Respuesta:

 

Product number

Description

Material

Swinging bucket rotor [x g]

Fixed angled rotor [x g]

186 1xx

15 ml, round bottom

polystyrene

2.000

2.500

188 1xx

15 ml, conical bottom

polystyrene

2.500

4.500

188 2xx

15 ml, conical bottom

polypropylene

4.000

15.000

210 2xx

50 ml, conical bottom, with skirt

polypropylene

2.800

11.500

227 261,
-270

50 ml, conical bottom

polypropylene

3.200

17.000

227 281,
-285

50 ml, conical bottom

polypropylene

3.200

9.500

227 280,
-283
50 ml, conical bottompolypropylene3.2009.000

Disclaimer:

The stated maximum RCF values are guidelines only, depending on a variety of factors such as rotor, temperature, density, pH and type of liquid. The suitability of tubes for a specific application using high centrifugation forces has to be tested.

Respuesta:

The PET roller bottles CAN NOT be shipped by air. They are sensitive to changes in air pressure and may implode if the cabin loses pressure. The polystyrene bottles can be shipped by air.

Respuesta:

Greiner Bio-One is offering various products for tissue- and cell culture:

1. CELLSTAR® products: These products can be used for general tissue- and cell culture. To facilitate cultivation of adherent cell a proprietary physical surface treatment is provided. These products are defined as TC-treated.

2. CELLCOAT® protein coated products: For the cultivation of sensitive cells as well as for specific experimental set-ups Greiner Bio-One  is offering Protein-coated cell culture vessels. The surface of these vessels is coated with proteins of the extracellular matrix as well as with synthetic polypeptides. Following protein coatings are available:
- Poly-D-Lysine
- Poly-L-Lysine
- Collagen I
- Laminin
- Fibronectin 

Respuesta:

Yes, the low basic resistance of the membrane of ThinCert™ cell culture inserts guarantees their excellent performance in TEER measurements.   

Respuesta:

In many cases the best results for organotypic culture have been obtained with ThinCert™ cell culture inserts with 0.4 µm and 1.0 µm. Sometimes 3.0 µm pores have been successfully applied.   

Respuesta:

Due to their low protein absorption PET membranes are very well suited for transport studies. For this kind of application the pores of the membrane must be small enough to prevent cells from membrane passage. ThinCertTM cell culture inserts with 0.4 and 1.0 µm pores meet this criterion. In general membranes with a high pore density (translucent) give better results than membranes with a low pore density (transparent).

Interesting Literature:
Bartholomé K, Rius M, Letschert K, Keller D, Timmer J, Keppler D. Kopplow K, Letschert K, Konig J, Walter B, Keppler D.
Data-Based Mathematical Modeling of Vectorial Transport across Double-Transfected Polarized Cells.
Drug Metab Dispos. 2007 Sep;35(9):1476-81.
Kopplow K, Letschert K, Konig J, Walter B, Keppler D.
Human hepatobiliary transport of organic anions analyzed by quadruple-transfected cells.
Mol Pharmacol. 2005 Oct;68(4):1031-8.
Epub 2005 Jul 26.

Letschert K, Faulstich H, Keller D, Keppler D.
Molecular characterization and inhibition of amanitin uptake into human hepatocytes.
Toxicol Sci. 2006 May;91(1):140-9. Epub 2006 Feb 22.

Letschert K, Komatsu M, Hummel-Eisenbeiss J, Keppler D.
Vectorial transport of the peptide CCK-8 by double-transfected MDCKII cells stably expressing the organic anion transporter OATP1B3 (OATP8) and the export pump ABCC2. J Pharmacol Exp Ther. 2005 May;313(2):549-56.
Epub 2005 Jan 21.

Mettlen M, Platek A, Van Der Smissen P, Carpentier S, Amyere M, Lanzetti L, de Diesbach P, Tyteca D, Courtoy PJ.
Src triggers circular ruffling and macropinocytosis at the apical surface of polarized MDCK cells.
Traffic. 2006 May;7(5):589-603.

Respuesta:

The thickness of the membrane of ThinCertTM cell culture inserts is graduated according to the size of the integrated pores. It is: 22.6 µm for 0.4 µm pores, 22 µm for 1.0 µm pores, 20 µm for 3.0 µm pores and 15 µm for 8.0 µm pores.   

Respuesta:

Transparent membranes comprise a lower pore density as compared to translucent membranes. With the different pore densities also the optical appearance changes. Transparent membranes are optimised for light microscopy.   

Respuesta:
  • PET is well established as a substrate for cell cultures.
  • PET is highly resistant against most organic solvents. When detached from the insert housing, the PET membrane does not curl. Due to high solvent resistance and anti-curling effects the membrane of ThinCertTM cell culture inserts is very well suited for subsequent processing, such as immunohistochemistry. >
  • PET exhibits a very low protein absorption. ThinCertTM cell culture inserts are therefore an excellent choice for transport and uptake studies.
Respuesta:

The capillary pore membrane of ThinCert™ cell culture inserts is made of PET (Polyethylene therephthalate).   

Respuesta:

The hanging geometry of ThinCert™ cell culture inserts ensures that a distance is kept between the insert underside and the well bottom, thus avoiding any damage of cells in the lower compartment during co-culture experiments. Furthermore, spacers at the flanges of the insert avoid any unwanted contact between the insert and the wall of the well.   

Respuesta:

The large opening for accessibility to pipettes is located opposite of the broad flange of the cell culture insert.   

Respuesta:

The eccentric position of ThinCert cell culture inserts conveniently provides a large opening for accessibility to pipettes, thus allowing an easy exchange of culture medium.   

Respuesta:

The external dimensions comply with ANSI Standards to render the flask suitable for use on a wide range of cell culture and liquid handling systems.    

Respuesta:

Poly-L- and Poly-D-Lysine are enantiomers and differ in the sterical assembly of the atoms in the molecule. Both coatings enhance adherence of cells in vitro. Poly-L-Lysine is in contrary to Poly-D-Lysine degradable by cellular proteases.

Respuesta:

Cat.-No. 633180 is a petri dish for bacteriology and not suitable for cell culture. As this dish has not a treated surface, cell adhesion is not possible. Furthermore, cell culture products like Cat. No. 633171 are sterile and regularly checked on cytoxity, freedom of RNase, DNase and human DNA and endotoxin.

Download PDF:  Quality Certificate

Respuesta:

Polypropylene has the distinction of high temperature-, high mechanical and high chemical resistance.   

Respuesta:

Polystyrene has the distinction of high transparency. Therefore it is suitable for all applications in which good optic is essential.   

Respuesta:

Yes, our petri dishes for bacteriology, like Cat.No. 663 102 or 664 102 are not pyrogen free. Our CELLSTAR®cell culture dishes are non-pryogenic, e.g. Cat. No. 664 160 (100 x 20 mm). On the website or in the catalogue our non-pyrogenic products are marked with a sign. The limit of detection for pyrogens is <= 0,06 EU/ml.

Respuesta:

The compartmented Petri dish enables working with different Agar Systems.

Respuesta:

Due to the heat resistance, the heavy version of the Petri Dish is suitable for applications with hot liquid agar.

Respuesta:

The shelf life of Petri dishes includes four years for TC-treated variants and 5 years for sterile, but not treated Petri dishes. The shelf life date of the product is printed on the outer label of the carton (hourglass) and is also located for cell culture products on the bag of each product.

Respuesta:

Yes, the thin-walled polystyrene is gas permeable.

Respuesta:

The spectrophotometric range of the semi-micro and macro cuvettes made from polystyrol refers to 370 - 750 nm.

Respuesta:

The bayonet cap can be opened by a short turn (1/3) of the cap, while the screw cap can be opened by a simple turn.

Respuesta:

Two-position vent stopper fulfils two functions:
1.When attached lightly, this enables a uniform ventilation of the tube contents.
2.When attached firmly by pressing in the stopper further, an airtight closure of the tube is achieved.

Respuesta:

The graduation of 15 ml- (177 xxx) and 50 ml-polypropylene tubes is precise in a tolerance range of +/- 2%.

Respuesta:

Centrifuge tubes as well as other products of polystyrene are not autoclaveable. Polystyrene is resistant to temperatures between -20 °C and +60 °C. Polystyrene products will deform themselves because of the high temperature (+120 °C) during the autoclaving process.

Respuesta:

 Yes, the following polycarbonate tubes are available

 

 
Product Number
Dimensions Volume Additional Feature
160 501 16,0 x 100 mm 12 ml  round bottom
172 501 16,5 x 103 mm 13 ml  conical bottom
169 501 16,0 x 152 mm 20 ml  round bottom
Respuesta:

Maximal centrifugation forces depend on the type of rotor and are specified as follows:

 

 
Product Description
Product Number max. RCF Fixed Angle Rotor [g] max. RCF Swinging Bucket Rotor [g]
15ml PP tube 188xxx 15.000 4.000
50ml PP tube 210xxx 11.500 2.800
50ml PP tube 227xxx 9.500 3.200
Respuesta:

Greiner Bio One does not offer a special rack for item 187262. It is recommended to use a standard tube rack for this product.

Respuesta:

All laboratories which manipulate potentially hazardous biological agents and materials, and generate waste containing such agents are responsible for the separation, packaging and treatment of their laboratory waste prior to its removal and disposal.

Greiner Bio One as supplier of disposable plastic ware cannot indicate the appropiate waste disposable the latter beeing dependent on the respective customer application and country-specific regulations. All Greiner plastic disposables are for single use only and cannot be recycled.

Please find below universal recommendations for biological waste disposal:

Biological waste includes:

     

  • liquids such as used cell culturing media, supernatant, blood or blood fractions (serum), etc., which contain viable biological agents;
  • materials considered pathological, including any part of the human body, tissues and bodily fluids, but excluding fluids, extracted teeth, hair, nail clippings and the like that are not infectious;
  • any part of an animal infected [or suspected to be infected] with a communicable disease;
  • non-sharp, solid laboratory waste (empty plastic cell culture flasks and petri dishes, empty plastic tubes, gloves, wrappers, absorbent tissues, etc.) which may be, or is known to be, contaminated with viable biological agents;
  • all sharp and pointed items used in medical care, diagnosis, and research, including the manipulation and care of laboratory animals, which should be considered potentially infectious;
  • laboratory glassware which is known or suspected to be contaminated with hazardous biological agents.

In addition to the general packaging requirements specific requirements for biological waste must be followed:

a) Liquids containing Biohazardous Agents

     

  • Collect liquids in leak-proof containers such as flasks or bottles.
  • Liquid waste containers designed to withstand autoclaving temperatures must be used when steam sterilization is utilized. To allow pressure equalization, they should not be sealed.

b) Solids Containing Biohazardous Agents 

     

  • Non-sharp, solid laboratory waste (empty plastic cell culture flasks and petri dishes, empty plastic tubes, gloves, wrappers, absorbent tissues, etc.) which may be, or is known to be, contaminated with viable biological agents should be collected in autoclavable bags. 

c) Sterilization and Disinfection

     

  • Autoclaving (steam sterilization) is the preferred (and generally regarded as the most reliable) method of sterilizing biological waste. Depending on the volume of waste to be sterilized, it may be necessary to extend the duration of exposure to high temperature steam under pressure.
  • Steam sterilization is generally not recommended for laboratory waste contaminated with or containing a combination of viable biological agents and significant amounts of hazardous chemical or radioactive materials.  
  • Containers of liquid waste must be placed into an autoclavable tray or pan of sufficient capacity to contain all liquid in the event of vessel failure or breakage inside the autoclave chamber. Use extreme caution when handling autoclaved liquids since they are hot and may boil over.
  • Autoclavable bags of solid waste should be closed but not sealed airtight to allow steam penetration before they are placed into the autoclave chamber.
Respuesta:

The maximum centrifuge capacity of PP-tube Cat. No. 187 261 is:  

  • Swinging bucket rotor max. 4800 RCF 
  • Fixed-angle rotor max. 34000 RCF
Respuesta:

Polypropylene tubes with the product number 188271 are UV-permeable. To store UV or light sensitive samples Greiner Bio-One offers so called "Light Protection Tubes".

Respuesta:

It is not recommended to use cell culture treated plates (TC treated microplates / CELLSTAR® microplates) in ELISAs. The cell culture treatment (TC treatment) of polystyrene creates highly charged surfaces with high affinity to all kind of polar proteins. Therefore the non specific background in the cell culture treated microplates is hard to control. Additionally, the ability of our immunological products  (MICROLON®) to bind proteins consistently and reproducibly is constantly checked in our quality assurance laboratory by means of ELISA.

Respuesta:

High binding polystyrene surfaces are hydrophilised using a special method of physical treatment. They display a higher binding capacity for polar molecules.
The polystyrene surface of a medium binding microplate is more hydrophobic than the surface of a high binding microplate and is therefore better suited for non-polar molecules.

Respuesta:

Greiner Bio-One offers microplates with two different F-Bottom formats - Standard F-Bottom and F-Bottom Chimney Well. Half Area is equal to the F-Bottom format, the smaller well size reduces the sample volume about 50 %.

F-Bottom Standard:
- Total Volume: 382 µl
- Working Volume: 25 - 340 µl

F-Bottom Chimney Well:
- Total Volume: 392 µl
- Working Volume: 25 - 340 µl

F-Bottom Half Area:
- Total Volume: 199 µl
- Working Volume: 15 - 175 µl

Respuesta:

LUMITRAC™ are white microplates. FLUOTRAC™ stands for black microplates. As the assay was developed in white high binding plates (LUMITRAC™ 600, 96 well) our recommendation would be to switch to white 384 well LUMITRAC™ microplates. The LUMITRAC™ 600 plate with the part number # 781074 is a white 384 well high binding microplate we would recommend.

Respuesta:

Yes, the Greiner UV Star microplates (# 655801, # 675801 and # 781801) should be compatible with the Varioskan microplate reader as they have a uniform footprint which is conform to the American National Standard Institute (ANSI / SBS Standards 1-2004). Detailed customer drawings with all necessary dimensions for the setup of a new plate definition file can be downloaded from our website www.gbo.com/bioscience under the equivalent part number.
These are the PDF-files of the customer drawings:
- 655801
- 675801
- 781801

Respuesta:

The 96 well µClear®microplates and 384 well µClear®microplates have a film thickness of 190 µm +/- 10%. In the 1536 well microplate with a transparent bottom (µClear®) the film thickness is 75 µm +/- 10%. UV-Star® microplates generally have a film thickness of 135 µm +/- 10 µm.