Ethical, scientific and financial concerns lead to an increasing importance of cell based assays and three-dimensional cell cultures in lieu of live-animal experimentation. With respect to these developments, ThinCert™ cell culture inserts with porous PET membranes are significant because they form a two-compartment system to readily mimic a variety of in vivo situations, such as:

1.  Cell migration and invasion,

2.  Interaction and communication of physically separated cell populations (co-culture)

3.  Trans-epithelial transport,

4.  Tissue growth and differentiation at the air-liquid-interface.

This presentation provides detailed insight in the different application fields of ThinCert™ cell culture inserts. Hints for the individual experimental design and the selection of the appropriate insert type are given.

During the course of this 60 minute, P.A.C.E.® accredited webinar, attendees will achieve the following program objectives:

1. Identify best practices in collection of non-blood specimens.

2. Review situations resulting in rejection of non-blood specimens.

3. Explain the potential patient impact caused by inadequate specimen collection.