Scaffold Free Magnetic 3D Methodologies Found to Be Better Suited for Analysis of ECM Protein Regulation

Monroe, North Carolina, May 2021 – 

A recent study published on May 12th, 2021 in Science Direct titled “Scaffold-free 3D cell culture of primary skin fibroblasts induces profound changes of the matrisome” showcases the difference in outcomes when scaffold-free methods are used for spheroid growth compared to scaffolded options when used on primary normal human fibroblasts. 

The published study used quantitative mass spectrometry-based proteomics in combination with magnetic 3D bioprinting and characterized changes in the proteome of skin fibroblasts and squamous cell carcinoma cells. It was determined that Transferring cells from 2D to 3D without any additional scaffold induces a profound upregulation of matrisome proteins indicating the generation of a complex, tissue-like extra-cellular matrix. 

Magnetic 3D bioprinting methodologies used in this study were a proprietary product line of products from Greiner Bio-One included in a 6-well Bio-Assembler Kit which includes a cell-repellent 6 well plate, magnetic concentrating drive, and magnetization of cells was achieved through magnetic nanoparticles known as NanoShuttle™.

The open-source study can be found in its entirety at Science Direct at the following location.;


About Magnetic 3D Cell Culture Products and Methods

The core technology of Greiner Bio-One’s Magnetic 3D Cell Culture is the magnetization of cells with biocompatible NanoShuttle™-PL. The reproducible formation of one spheroid per well in an F-bottom plate with Cell-Repellent surface is forced by magnets either by levitation or bioprinting, to form structurally and biologically representative 3D models in-vitro. For more information regarding these products, please visit